Abstract
Since ancient times, medicinal plants have been utilized to cure and prevent a wide range of animal and human illnesses. Nowadays, the use of therapeutic herbs is increasing in utilization of medicinal plants. The growing resistance of bacteria to antibiotics has made humans more worried about finding other ways to cure infections. Phytoconstituents have the potential to act as antimicrobial drugs against sensitive and resistant pathogens by various modes of action. Phytobiotics are naturally occurring bioactive compounds derived from plants that are secondary metabolites such as essential oils, phenolics, flavonoids, tannins, alkaloids, organosulfur, and saponins. These compounds are produced by herbal plants and have antimicrobial and growth-promoting properties that can help treat a variety of animal diseases. Plant extracts possess several advantages over synthetic drugs as most of them have no residual effects, due to its applicability in human medicine so they could be added to animals’ food to prevent and treat various animal health disorders in addition to their possible nutritional characteristics. However, additional investigation is necessary to have a greater awareness of the phytochemicals’ mechanisms of action. In this regard, this review summarizes several effects of some biologically active plants and their phytoconstituents on some infectious microbial diseases in animal. It also summarizes the efficiency, modes of action, and some applications of medicinal plants active phytochemicals to solve the antibiotic resistance problem. Regarding these facts, it could be concluded that medicinal plants resemble a furious source of active constituents that possess antimicrobial potential.
Keywords: Phytoconstituents · phenolics · flavonoids · tannins · terpenes · alkaloids · essential oils · polypeptides
References
- 1.Shin B,Park W.Zoonotic diseases and phytochemical medicines for microbial infections in veterinary science: current state and future perspective. Front Vet Sci. 2018;5:166.
- 2.Kessler CS, Perera PK, Puthiyedath R, Dhruva A. Editorial: The increasing relevanceoftraditionalmedicinesystemsfor the primary health care sector and general practice:global research perspectives.Vol. 11,Frontiers in Medicine.Frontiers Media SA;2024.p.1533361.
- 3.Parvin S, Reza A, Das S, Miah MMU, Karim S.Potential Role and International Trade of Medicinal and Aromatic Plants 258 Majmaah Journal of Health Sciences,Vol.14, Issue2, April-2026,Shawwal-1447 Mode of actions of some phytoconstituentsofmedicinalplantsincontrollinganimalmicrobialdisease in the World. Eur J Agric Food Sci. 2023;5:89–99.
- 4.Rezzoug M, Bakchiche B, Gherib A, Roberta A,Guido F,Kilinçarslan Ö,et al.Chemical composition and bioactivity of essential oils and Ethanolic extracts of Ocimum basilicum L. and Thymus algeriensisBoiss.&Reut.fromtheAlgerian Saharan Atlas.BMC Complement Altern Med.2019;19:1–10.
- 5.Njoroge GN, Bussmann RW. Herbal usage and informant consensus in ethnoveterinary management of cattle diseases among the Kikuyus (Central Kenya).JEthnopharmacol.2006;108:332–9.
- 6.Confessor MVA,Mendonça LET,Mourão JS,Alves RRN.Animals to heal animals: Ethnoveterinary practices in semiarid region,Northeastern Brazil.J Ethnobiol Ethnomed.2009;5:1–9.
- 7.Karimi A, Majlesi M, Rafieian-Kopaei M. Herbal versus synthetic drugs; beliefs and facts.J nephropharmacology [Internet]. 2015;4:27–30. Available from: http://www.ncbi.nlm.nih.gov/ pubmed/28197471%0Ahttp://www. pubmedcentral.nih.gov/articlerender. fcgi?artid=PMC5297475
- 8.Salama MM, Ezzat SM, Salem MA. Bioactive lead compounds and molecular targets for the treatment of heart diseases. Phytochem as Lead Compd New Drug Discov.2019;67–94.
- 9.37.Russell W, Duthie G. Plant secondary metabolites and gut health: the case for phenolicacids.ProcNutrSoc.2011;70:389–
- 10.Ni ZJ,Wang X, Shen Y,Thakur K, Han J,Zhang JG,et al.Recent updates on the chemistry,bioactivities,mode of action,and industrial applications of plant essential oils.Trends Food Sci Technol [Internet]. 2021;110:78–89. Available from: https://www.sciencedirect.com/ science/article/pii/S0924224421000789
- 11.Sarowska J,Wojnicz D,Jama-Kmiecik A, Frej-Mądrzak M, Choroszy-Król I. Antiviral potential of plants against noroviruses. Molecules [Internet]. 2021;26. Available from: https://www. mdpi.com/1420-3049/26/15/4669
- 12.Perez G. RM. Antiviral activity of compounds isolated from plants.Pharm Biol. 2003;41:107–57.
- 13.AllahverdiyevA,DuranN,OzguvenM, Koltas S.Antiviral activity of the volatile oils of Melissa officinalis L.against Herpes simplex virus type-2. Phytomedicine. 2004;11:657–61.
- 14.Koch C, Reichling J, Schnitzler P. Essential oils inhibit replication of herpes simplex virus type 1 and type 2.Bot Med Clin Pract.2017;192:192–7.
- 15.Baqui AAMA, Jabra-Rizk MA, DePaola LG, Falkler WA, Meiller TF. 266 Majmaah Journal of Health Sciences,Vol.14, Issue2, April-2026,Shawwal-1447 Mode of actions of some phytoconstituentsofmedicinalplantsincontrollinganimalmicrobialdisease In vitro effect of oral antiseptics on human immunodeficiency virus-1 and herpes simplex virus type 1.J Clin Periodontol. 2001;28:610–6.
- 16.PrimoV,RoveraM,ZanonS,OlivaM, Demo M,Daghero J,et al.Determination of the antibacterial and antiviral activity of the essential oil from Minthostachys verticillata (Griseb) Epling.Rev Argent Microbiol.2001;33:113–7.
- 17.Gupta NS.Therapeutic Efficacy of the Plant Bioactive Phytochemicals with SpecialReferencetoAlkaloids,Terpenoids, Phenolics and Cardiac Glycosides.Int J Plant Environ [Internet]. 2024;10:22– 30.Available from:https://ijplantenviro. com/index.php/IJPE/article/view/1736
- 18.Yadav K, Divyadeepika, Joshi J. Biological activity of phytochemicals extracted from medicinal plants of Apocynaceae family.MaterToday Proc. 2024;
- 19.Domadia PN,Bhunia A,Sivaraman J, Swarup S,Dasgupta D.Berberine targets assembly of Escherichia coli cell division proteinFtsZ.Biochemistry.2008;47:3225–
- 20.Schrader KK, Avolio F, Andolfi A, Cimmino A, Evidente A. Ungeremine and its hemisynthesized analogues as bactericides against flavobacterium columnare. J Agric Food Chem. 2013;61:1179–83.
- 21.Qi J,Zhang X,Guo X,YangY,Fan X, HanY,et al.Evaluation of the in vitro and in vivo antimicrobial activity of alkaloids prepared from Chelidonium majus L. using MRSA- infected C. elegans as a modelhost.Fitoterapia.2024;175:105944.
- 22.Dusane DH, Hosseinidoust Z, Asadishad B, Tufenkji N. Alkaloids modulate motility, biofilm formation and antibiotic susceptibility of uropathogenic Escherichia coli. PLoS One. 2014;9:e112093.
- 23.Dwivedi GR, Gupta S, Maurya A, Tripathi S, Sharma A, Darokar MP, et al.Synergy potential of indole alkaloids and its derivative against drug-resistant Escherichia coli.Chem Biol Drug Des. 2015;86:1471–81.
- 24.Liu ZH,Wang WM,Zhang Z,Sun L, Wu SC. Natural Antibacterial and Antivirulence Alkaloids From Macleaya cordata Against Methicillin-Resistant Staphylococcus aureus.Front Pharmacol. 2022;13:813172.
- 25.Avci FG,Atas B,Gulsoy Toplan G, Gurer C,Sariyar Akbulut B.Antibacterial and antifungal activities of isoquinoline alkaloids of the Papaveraceae and Fumariaceaefamiliesandtheirimplications in structure–activity relationships.Stud Nat Prod Chem.2021;70:87–118.
- 26.Alghazeer R,Azwai S,Garbaj AM, Amr A, Elghmasi S, Sidati M, et al. Majmaah Journal of Health Sciences,Vol.14, Issue2, April-2026,Shawwal-1447 267 Mode of actions of some phytoconstituentsofmedicinalplantsincontrollinganimalmicrobialdisease Alkaloids rich extracts from brown algae against multidrug-resistant bacteria by distinctive mode of action. Arab J Sci Eng. 2022;1–10.
- 27.EvansSM,CowanMM.Plantproducts as antimicrobial agents. Cosmet Drug Microbiol.2016;12:205–31.
- 28.Redondo LM, Dominguez JE, Rabinovitz BC,Redondo EA,Fernández Miyakawa ME. Hydrolyzable and condensedtanninsresistanceinClostridium perfringens. Anaerobe [Internet]. 2015;34:139–45.Available from:https:// www.sciencedirect.com/science/article/ pii/S1075996415300287
- 29.Frutos P, Hervás G, Giráldez FJ, Mantecón AR. Review. Tannins and ruminant nutrition.Spanish J Agric Res. 2004;2:191.
- 30.Tong Z, He W, Fan X, Guo A. Biological Function of PlantTannin and Its Application in Animal Health.Front Vet Sci. 2022;8:1–7.
- 31.Kaczmarek B. Tannic acid with antiviral and antibacterial activity as a promising component of biomaterials-A minireview.Materials (Basel) [Internet]. 2020;13. Available from: https://www. mdpi.com/1996-1944/13/14/3224
- 32.Belhaoues S,Amri S,Bensouilah M. Major phenolic compounds,antioxidant and antibacterial activities of Anthemis praecox Link aerial parts.South African J Bot [Internet].2020;131:200–5.Available from: https://www.sciencedirect.com/ science/article/pii/S0254629919322677
- 33.Vance SH,Tucci M, Benghuzzi H. Evaluation of the antimicrobial efficacy of green tea extract (EGCG) against streptococcus pyogenes in vitro.Biomed Sci Instrum [Internet].2011;47:177–82. Available from: http://europepmc.org/ abstract/MED/21525617
- 34.Pandey A, Negi PS. Phytochemical composition,invitroantioxidantactivityand antibacterial mechanisms of Neolamarckia cadamba fruits extracts. Nat Prod Res [Internet].2018;32:1189–92.Available from:https://doi.org/10.1080/1478641 9.2017.1323209 doi:10.1080/1478641
- 35.Wina E, Muetzel S, Becker K.The impact of saponins or saponin-containing plant materials on ruminant production - A review.J Agric Food Chem [Internet]. 2005;53:8093–105.Availablefrom:https:// doi.org/10.1021/jf048053d doi:10.1021/jf048053d
- 36.PatraAK,SaxenaJ.Theeffectandmode of action of saponins on the microbial populationsandfermentationintherumen and ruminant production.Nutr Res Rev. 2009;22:204–19.
- 37.Rosen JD.Saponins.K.Hostettmann and A.Marston.Analytical Biochemistry. Cambridge [etc:Cambridge Univ.Press; 1996.119p.(Chemistryandpharmacology of natural products;vol.239). 268 Majmaah Journal of Health Sciences,Vol.14, Issue2, April-2026,Shawwal-1447 Mode of actions of some phytoconstituentsofmedicinalplantsincontrollinganimalmicrobialdisease
- 38.Vincken JP, Heng L, de Groot A, Gruppen H. Saponins, classification and occurrence in the plant kingdom. Phytochemistry [Internet].2007;68:275–
- 39.Available from: https://www. sciencedirect.com/science/article/pii/ S0031942206006480
- 40.Ibrahim NA,Rathore D,Janiyani K, Gupta A,Sulieman AME,Tahir HE,et al. A comprehensive review on plantderived bioactive saponins as promising antimicrobial agents:from bioavailability challenges,molecular mechanistic insights to therapeutic applications. Naunyn SchmiedebergsArchPharmacol.2025;1–31.
- 41.Lanzotti V, Scala F, Bonanomi G. Compounds from Allium species with cytotoxic and antimicrobial activity. Phytochem Rev. 2014;13:769–91.
- 42.Bhatwalkar SB,Mondal R,Krishna SBN,Adam JK,Govender P,Anupam R. Antibacterial Properties of Organosulfur Compounds of Garlic (Allium sativum) [Internet]. Vol. 12, Frontiers in Microbiology.2021.Availablefrom:https:// www.frontiersin.org/article/10.3389/ fmicb.2021.613077
- 43.Khare T, Anand U, Dey A, Assaraf YG, Chen ZS, Liu Z, et al. Exploring Phytochemicals for Combating Antibiotic Resistance in Microbial Pathogens.Front Pharmacol.2021;12.
- 44.Lu Z, Dockery CR, Crosby M, Chavarria K, Patterson B, Giedd M. Antibacterial activities of wasabi against Escherichia coli O157: H7 and Staphylococcus aureus.Front Microbiol. 2016;7:1403.
- 45.Nedorostova L,Kloucek P,Kokoska L,Stolcova M,Pulkrabek J.Antimicrobial propertiesofselectedessentialoilsinvapour phase against foodborne bacteria.Food Control.2009;20:157–60.
Peer review
Peer reviewed by 4 reviewers, who chose to remain anonymous.